Journal: The Journal of Physical Chemistry. C, Nanomaterials and Interfaces

Article Title: Texture or Linker? Competitive Patterning of Receptor Assembly toward Ultra-Sensitive Impedimetric Detection of Viral Species at Gold-Nanotextured Titanium Surfaces

doi: 10.1021/acs.jpcc.3c00697

Figure Lengend Snippet: (A) electrochemical cell and (B) AuTi-NTE electrode photographs. Topography of as-prepared AuTi-NTE, visualized by: (C) AFM ( D) FE-SEM. (E–H) functionalization scheme: (E) unmodified AuTi-NTE, (F) MPA adsorption, (G) consecutive functionalization steps, (H) ready-to-use sensor with CD147 anchored. (I) XPS S2p analysis after modification with MPA.

Article Snippet: The biological materials, i.e., the receptor-transmembrane glycoprotein CD147 (972-EMN) (CD147), and the targeted SARS-CoV-2 Spike receptor-binding domain proteins (CHO: 1-534-CV) (RBD), were obtained from R&D Systems.

Techniques: Adsorption, Modification

Journal: The Journal of Physical Chemistry. C, Nanomaterials and Interfaces

Article Title: Texture or Linker? Competitive Patterning of Receptor Assembly toward Ultra-Sensitive Impedimetric Detection of Viral Species at Gold-Nanotextured Titanium Surfaces

doi: 10.1021/acs.jpcc.3c00697

Figure Lengend Snippet: Adsorption of the CD147 receptor on the gold surface with MPA-EDC_NHS functionalization. (A) Geometry of the CD147 receptor on the Au surface in two perpendicular projections—the tilt of the protein is marked black and equal to ca. 60°; (B and C) schematic illustration of 9 CD147 receptors on the flat gold and AuNP surfaces. Green segments indicate the place at the receptor for binding of the viral protein.

Article Snippet: The biological materials, i.e., the receptor-transmembrane glycoprotein CD147 (972-EMN) (CD147), and the targeted SARS-CoV-2 Spike receptor-binding domain proteins (CHO: 1-534-CV) (RBD), were obtained from R&D Systems.

Techniques: Adsorption, Binding Assay

Journal: Endocrine-Related Cancer

Article Title: Interleukin-6 trans-signalling differentially regulates proliferation, migration, adhesion and maspin expression in human prostate cancer cells

doi: 10.1677/erc-09-0200

Figure Lengend Snippet: Figure 1 Addition of sgp130 reveals an anti-proliferative effect of IL-6TS in LNCaP and Du145 cells. LNCaP (A), Du145 (B) and LNCaP-HP (C) cells were treated with increasing doses of sgp130, as indicated, and exposed to IL-6. Proliferation was measured by 3H-thymidine incorporation and values, expressed as meanGS.E.M., were referred as a percentage of vehicle- treated samples. (D) The effect of IL-6 on LNCaP-HP cell proliferation was assessed in the absence or presence of increasing doses of sgp130. Values, expressed as mean GS.E.M., are referred as a percentage of IL-6-untreated samples *P!0.05; **P!0.01; ***P!0.001.

Article Snippet: Recombinant human IL-6 and gp130/Fc Chimera (i.e. sgp130) were purchased from R&D Systems (Wiesbaden, Germany), and CD126 (sIL-6R) was www.endocrinology-journals.org purchased from PromoKine (Heidelberg, Germany).

Techniques:

Journal: Endocrine-Related Cancer

Article Title: Interleukin-6 trans-signalling differentially regulates proliferation, migration, adhesion and maspin expression in human prostate cancer cells

doi: 10.1677/erc-09-0200

Figure Lengend Snippet: Figure 5 IL-6TS increases cell motility. Cells were seeded onto cell culture inserts, as described in materials and methods. LNCaP (A) and Du145 (B) were exposed to IL-6 after treatment with sgp130 or vehicle. LAPC4 (C) were exposed to IL-6 in the presence or absence of 100 ng/ml sIL-6R. Motility was measured by counting cells that migrated through the membrane. Scale bar is 200 mm. Values are expressed as meanGS.E.M. *P!0.05; **P!0.01; ***P!0.001.

Article Snippet: Recombinant human IL-6 and gp130/Fc Chimera (i.e. sgp130) were purchased from R&D Systems (Wiesbaden, Germany), and CD126 (sIL-6R) was www.endocrinology-journals.org purchased from PromoKine (Heidelberg, Germany).

Techniques: Cell Culture, Membrane

Journal: Endocrine-Related Cancer

Article Title: Interleukin-6 trans-signalling differentially regulates proliferation, migration, adhesion and maspin expression in human prostate cancer cells

doi: 10.1677/erc-09-0200

Figure Lengend Snippet: Figure 7 Addition of sIL-6R and IL-6 decreases adhesion to fibronectin, which can be inhibited by sgp130. Du145 cells were seeded on fibronectin substrate in the presence of sgp130 or vehicle and treated with IL-6 in the absence or presence of sIL-6R. Values are expressed as meanGS.E.M. **P!0.01.

Article Snippet: Recombinant human IL-6 and gp130/Fc Chimera (i.e. sgp130) were purchased from R&D Systems (Wiesbaden, Germany), and CD126 (sIL-6R) was www.endocrinology-journals.org purchased from PromoKine (Heidelberg, Germany).

Techniques:

Journal: Endocrine-Related Cancer

Article Title: Interleukin-6 trans-signalling differentially regulates proliferation, migration, adhesion and maspin expression in human prostate cancer cells

doi: 10.1677/erc-09-0200

Figure Lengend Snippet: Figure 9 Maspin expression is decreased by IL-6. This effect can be antagonised by the addition of sgp130. Cells were treated for 48 h with or without IL-6 in the absence or presence of sgp130, as indicated. Maspin and GAPDH (loading control) expression levels were analysed by western blotting and quantified densitometrically. Values are expressed as mean GS.E.M. *P!0.05.

Article Snippet: Recombinant human IL-6 and gp130/Fc Chimera (i.e. sgp130) were purchased from R&D Systems (Wiesbaden, Germany), and CD126 (sIL-6R) was www.endocrinology-journals.org purchased from PromoKine (Heidelberg, Germany).

Techniques: Expressing, Control, Western Blot